Keywords
Clareamento Dental
Esmalte Dental
Microhardness
CPP-ACP
NovaMin®. Dental Whitening
Dental Enamel
Microdureza
CPP-ACP
NovaMin®.
Esmalte Dental
Microhardness
CPP-ACP
NovaMin®. Dental Whitening
Dental Enamel
Microdureza
CPP-ACP
NovaMin®.
How to Cite
Nahum, A. L., Gomes, Y. do S. B. de L., Pompeu, D. da S., Ortiz, M. I. G., Emmi, D. T., & Silva, C. M. (2018). EFEITO IN SITU DE AGENTES BIOATIVOS NO ESMALTE: IN SITU EFFECT OF BIOACTIVE AGENTS ON ENAMEL. APO Digital Journal, 2(1), 30–36. https://doi.org/10.5935/2526-8155.20180004
Abstract
This study evaluated in situ the effects of bioactive agents on enamel whitened with hydrogen peroxide at 37.5%, using Knoop microhardness (KHN) and ion chromatography (IC). We selected25 third human molars included were fragmented (3x3x3 mm). Five volunteers were selected and had five specimens attached to the buccal surface of their upper and lower molars. Each volunteer represented a group (n = 5): CONTROL: no treatment, POLA: Pola Office 37.5% (Positive Control), NANO: Pola Office 37.5% + Nanohydroxyapatite, CPP- + CPP-ACP (CPP-ACP) and NOVA: Pola Office 37,5% + NovaMin. KHN analysis (n = 5) was performed before and after treatments. Five indentations were performed, using the 25 gf load for 15 seconds. For the chemical analysis (n = 3), the samples were ground, autoclaved and subjected to acid digestion by microwave radiation. They were then diluted in 303x with distilled / deionized water. To quantify the chemical elements calcium (Ca), fluorine (F) and phosphorus (P), an ion chromatography technique was used. ANOVA followed by TUKEY (p <0.05) showed that the CPP-ACP and NOVA groups showed higher KHN means in relation to the other groups; the NANO group was statistically similar to the CONTROL group; The POLA presented the lowest KHN mean when compared to the other groups. The percentage of KHN in descending order was: CPP-ACP, NOVA, NANO CONTROLE and POLA. The use of bioactive agents in the bleaching treatment promoted an increase in the percentage of calcium, fluorine and phosphorus ions and the hardness of human enamel.
References
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[30] Hui-Ping C.Effect of fluoride containing bleaching agents on enamel surface properties. J. Dent. 2008; 36: 718–725.
[2] Gruber A, Colli L, Muniz C, Ottoni R, Borba M. Efeito do uso de agentes clareadores com cálcio na microdureza do esmalte dental. Revista da Faculdade de Odontologia-UPF. 2017; 22(3).
[3] Sa Y, Chen D, Liu Y, Wen W, Xu M, Jiang T, Wang Y. Effects of two in-office bleaching agents with different pH values on enamel surface structure and color: An in situ vs. in vitro study.J. Dent. 2012; 40: 26-34.
[4] Zeczkowski M, Tenuta LM, Ambrosano, GM, Aguiar FH, Lima DA. Effect of different storage conditions on the physical properties of bleached enamel: an in vitro vs in situ study. J. Dent. 2015; 43 (9): 1154–1161.
[5] Margaritis V, Mamai-Homata E, Koletsi-Kounari H. Novel methods of balancing covariates for the assessment of dental erosion: a contribution to validation of a synthetic scoring system for erosive wear. J. Dent. 2011; 39: 361–7.
[6] Borges AB, Yui KC, D’Avila TC, Takahashi CL, Torres CR, Borges AL. Influence of remineralizing gels on bleached enamel microhardness in different time intervals. Oper. Dent. 2010; 35 (2):180–186.
[7] Cakir FY, Korkmaz Y, Firat E, Oztas SS, Gurgan S. Chemical analysis of enamel and dentin following the application of three different at-home bleaching systems.Oper. Dent. 2011; 36 (5): 529–536.
[8] Moraes I, Silva L, Porto I, Lima Neto C, Santos N, Fragoso L. Effect of in-office bleaching with 35% hydrogen peroxide with and without addition of calcium on the enamel surface. Microsc. Res. Tech. 2015; 78 (11): 975–981.
[9] Pintado-Palomino K,Peitl Filho O,Zanotto ED,Tirapelli CA. Clinical, randomized, controlled study on the use of desensitizing agents during tooth bleaching. J. Dent. 2015; 43 (9): 1099-1105.
[10] Wang Z, Jiang T, Sauro S, Pashley Dh, Toledano M, Osorio R, Liang S, Xing W, Sa Y, Wang Y. The dentine remineralization activity of a desensitizing bioactive glass-containing toothpaste: an in vitro study. Aust. Dent. J. 2011; 56: 372–381.
[11] Tschoppe P, Zandim, DL, Martus P,Kielbassa AM. Enamel and dentine remineralization by nano-hydroxyapatite toothpastes. J. Dent. 2011, 39: 430–437.
[12] De Vasconcelos M, Cunha G, Borges D, Machado T, Dos Santos S. Tooth whitening with hydrogen/carbamide peroxides in association with a CPP-ACP paste at different proportions. Aust. Dental. J. 2012; 57: 213–219.
[13] Yamamoto T W, de Carvalho RCR. Efeito da utilização de dentifrícios com diferentes compostos bioativos nas propriedades superficiais do esmalte dental clareado. Revista de Odontologia da Universidade Cidade de São Paulo. 2017; 25 (2): 154-163.
[14] Smidt A, Topel M. Mechanical, morphologic, and chemical effects of carbamide peroxide bleaching agents on human enamel in situ. Quintessence. 2011; 42: 407– 411.
[15] Gjorgievska E, Nicholson JW. Prevention of enamel demineralization after tooth bleaching by bioactive glass incorporated into toothpaste. Aust. Dent. J. 2011; 56: 193–200.
[16] Ferreira SDS, Araújo JLN, Morhy ON, Tapety CMC, Youssef M, Sobral MAP. The effect of fluoride therapies on the morphology of bleached human dental enamel. Mic. Res. Tech. 2011; 74: 512-516.
[17] BorgesAB, SantosLFTF,Augusto MG, BonfietteD, Hara AT,Torres CRG.Toothbrushing abrasion susceptibility of enamel and dentin bleached with calcium-supplemented hydrogen peroxide gel. J. Dent. 2016; 49: 54-59.
[18] LeGeros RZ. Calcium phosphate materials in restorative dentistry: a review. Advan. Dent. Res. 1988; 2: 164–80.
[19] Sun L, Berndt CC, Gross KA. Material fundamentals and clinical performance of plasma-sprayed hydroxyapatite coatings: a review. J. Bio. Mat. Res. 2001; 58: 570–92.
[20] Huang, S.; Gao, S.; Cheng, L. & Yu, H. Remineralization potential of nanohydroxyapatite on initial enamel lesions: an in vitro study. Caries Res. 2011; 45: 460–468.
[21] Yu J, YangH, LiK, LeiJ, ZhouL, Huang C. A novel application of nanohydroxyapatite/mesoporous silica biocomposite on treating dentin hypersensitivity: An in vitro study. J. Dent. 2016; 50: 21–29.
[22] Kim YS, Kwon HK, Kim BI. Effect of nano-carbonate apatite to prevent re-stain after dental bleaching in vitro. J. Dent. 2011; 39: 636–64.
[23] Reynolds EC. Casein phosphopeptide-amorphous calcium phosphate: the scientific evidence. Adv. Dent. Res. 2009; 21: 25-9.
[24] Ruchi G, VijayP. CCP-ACP Complex as a new adjunctive agent for remineralisation.A review. Oral Health Prev. Dent. 2011; 9: 151-65.
[25] Mastroberardino S, Campus G,Strohmenger L. An innovative approach to treat incisors hypomineralization (MIH): a combined use of casein phosphopeptide-amorphous calcium phosphate and hydrogen peroxide - a case report. Case. Rep. Dent. 2012; 2012.
[26] Neuhaus KW, Milleman JL, Milleman KR.Effectiveness of a calcium sodium phosphosilicate containing prophylaxis paste in reducing dentine hypersensitivity immediately and 4 weeks after a single application: a double-blind randomized controlled trial. J. Clin. Period. 2013; 40: 349-357.
[27] Cavalli V, Rodrigues LK, Paes-Leme AF. Effects of the addition of fluoride and calcium to low-concentrated carbamide peroxide agents on the enamel surface and subsurface.Photomed.Laser. Surg. 2011; 29: 319-25.
[28] Farmakis E, Kozyrakis K, Khabbaz M,Beer U, Moritz A. In Vitro Evaluation of Dentin Tubule Occlusion by Denshield and Neodymium-doped Yttrium-Aluminum-Garnet Laser Irradiation. J. Endodontcs. 2012; 38(5): 662–666.
[29] Hao Y,Li Q, Hussain M. Effects of bleaching gels on the surface microhardness of tooth-colored restorative materials in situ. J. Dent. 2008; 36: 26–267.
[30] Hui-Ping C.Effect of fluoride containing bleaching agents on enamel surface properties. J. Dent. 2008; 36: 718–725.
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